Loop Free-Energy Decomposition Dimer 1 ΔG = -42.8 kcal/mol ΔH = -203.9 kcal/mol ΔS = -519.4 e.u. Tm= 100.9°CStructural elementδGInformation External loop +0.00 0 ss bases & 1 closing helices Stack -2.90 External closing pair is C 1 -G 19 Stack -2.90 External closing pair is C 2 -G 18 Stack -1.70 External closing pair is C 3 -G 17 Stack -2.30 External closing pair is U 4 -A 16 Stack -2.90 External closing pair is C 5 -G 15 Stack -2.90 External closing pair is C 6 -G 14 Stack -1.70 External closing pair is C 7 -G 13 Stack -2.30 External closing pair is U 8 -A 12 Stack -2.90 External closing pair is C 9 -G 11 Stack -2.00 External closing pair is C 10 -G 10 Stack -3.40 External closing pair is G 11 -C 9 Stack -2.90 External closing pair is C 12 -G 8 Stack -2.90 External closing pair is C 13 -G 7 Stack -2.00 External closing pair is C 14 -G 6 Stack -3.40 External closing pair is G 15 -C 5 Stack -2.90 External closing pair is C 16 -G 4 Stack -2.90 External closing pair is C 17 -G 3 Helix -44.90 18 base pairs External loop +2.10 Closing pair is C 18 -G 2 Dimer 2 ΔG = -43.3 kcal/mol ΔH = -206.0 kcal/mol ΔS = -524.6 e.u. Tm= 101.2°CStructural elementδGInformation External loop +0.00 0 ss bases & 1 closing helices Stack -2.90 External closing pair is C 1 -G 19 Stack -1.70 External closing pair is C 2 -G 18 Stack -2.30 External closing pair is U 3 -A 17 Stack -2.90 External closing pair is C 4 -G 16 Stack -2.90 External closing pair is C 5 -G 15 Stack -1.70 External closing pair is C 6 -G 14 Stack -2.30 External closing pair is U 7 -A 13 Stack -2.90 External closing pair is C 8 -G 12 Stack -2.00 External closing pair is C 9 -G 11 Stack -3.40 External closing pair is G 10 -C 10 Stack -2.90 External closing pair is C 11 -G 9 Stack -2.90 External closing pair is C 12 -G 8 Stack -2.00 External closing pair is C 13 -G 7 Stack -3.40 External closing pair is G 14 -C 6 Stack -2.90 External closing pair is C 15 -G 5 Stack -2.90 External closing pair is C 16 -G 4 Stack -2.00 External closing pair is C 17 -G 3 Stack -3.40 External closing pair is G 18 -C 2 Helix -47.40 19 base pairs External loop +4.10 Closing pair is C 19 -G 1 Dimer 3 ΔG = -43.3 kcal/mol ΔH = -206.0 kcal/mol ΔS = -524.6 e.u. Tm= 101.2°CStructural elementδGInformation External loop +0.00 0 ss bases & 1 closing helices Stack -1.70 External closing pair is C 1 -G 19 Stack -2.30 External closing pair is U 2 -A 18 Stack -2.90 External closing pair is C 3 -G 17 Stack -2.90 External closing pair is C 4 -G 16 Stack -1.70 External closing pair is C 5 -G 15 Stack -2.30 External closing pair is U 6 -A 14 Stack -2.90 External closing pair is C 7 -G 13 Stack -2.00 External closing pair is C 8 -G 12 Stack -3.40 External closing pair is G 9 -C 11 Stack -2.90 External closing pair is C 10 -G 10 Stack -2.90 External closing pair is C 11 -G 9 Stack -2.00 External closing pair is C 12 -G 8 Stack -3.40 External closing pair is G 13 -C 7 Stack -2.90 External closing pair is C 14 -G 6 Stack -2.90 External closing pair is C 15 -G 5 Stack -2.00 External closing pair is C 16 -G 4 Stack -3.40 External closing pair is G 17 -C 3 Stack -2.90 External closing pair is C 18 -G 2 Helix -47.40 19 base pairs External loop +4.10 Closing pair is C 19 -G 1 Dimer 4 ΔG = -43.6 kcal/mol ΔH = -206.3 kcal/mol ΔS = -524.6 e.u. Tm= 101.8°CStructural elementδGInformation External loop +0.00 0 ss bases & 1 closing helices Stack -2.30 External closing pair is U 1 -A 19 Stack -2.90 External closing pair is C 2 -G 18 Stack -2.90 External closing pair is C 3 -G 17 Stack -1.70 External closing pair is C 4 -G 16 Stack -2.30 External closing pair is U 5 -A 15 Stack -2.90 External closing pair is C 6 -G 14 Stack -2.00 External closing pair is C 7 -G 13 Stack -3.40 External closing pair is G 8 -C 12 Stack -2.90 External closing pair is C 9 -G 11 Stack -2.90 External closing pair is C 10 -G 10 Stack -2.00 External closing pair is C 11 -G 9 Stack -3.40 External closing pair is G 12 -C 8 Stack -2.90 External closing pair is C 13 -G 7 Stack -2.90 External closing pair is C 14 -G 6 Stack -2.00 External closing pair is C 15 -G 5 Stack -3.40 External closing pair is G 16 -C 4 Stack -2.90 External closing pair is C 17 -G 3 Helix -45.70 18 base pairs External loop +2.10 Closing pair is C 18 -G 2